An isotopic study of the conversion of anthranilic acid to indole.
نویسنده
چکیده
Anthranilic acid and indole have been implicated as intermediates in the biosynthesis of tryptophan in several microorganisms (l-5). A mechanism of tryptophan synthesis from indole has been established, namely the pyridoxal phosphate-dependent coupling of indole and L-serine (6-9). Previous studies of the conversion of anthranilic acid to the indole ring of tryptophan have shown that the amino nitrogen of anthranilic acid is retained (10) while the carboxyl carbon is removed (11). The actual steps in the conversion of anthranilic acid to indole, however, are at present unknown. Since the carboxyl carbon of anthranilic acid is lost during indole formation, 2 additional carbon atoms must be supplied to complete the pyrrole ring of indole. In recent experiments with extracts of Escherichia coli it was observed that various ribose derivatives would serve as the source of these 2 carbon atoms (12). The isotope experiments reported in this paper represent another approach to the problem of identifying the donor of the required carbon atoms. The data obtained suggest that the 2 carbon atoms in question are derived from carbons 1 and 2 of glucose by one pathway and from carbons 2 and 3 of glucose by a second pathway.
منابع مشابه
The use of isotopic carbon in a study of the metabolism of anthranilic acid in Neurospora.
The finding by Tatum, Banner, and Beadle (l), that the tryptophanless Neurospora mutant strain 10575 accumulates anthranilic acid, which in turn can be utilized for growth of strain 40008, has provided evidence that anthranilic acid is a biochemical precursor of tryptophan in this organism. It has been further established that indole is an intermediate in this conversion (2-5). More recent work...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 217 1 شماره
صفحات -
تاریخ انتشار 1955